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Challenge inoculation and assessment of disease
As per the results, the samples of both the cultivars, obtained from elicitor treated plants showed a delay in the disease initiation and reduction in the disease incidence and severity. In cv. HYW, the disease initiation took place on the 5th day after challenge inoculation with C. acutatum in elicitor treated plants, whereas control samples showed the diseases initiation on 4th day after inoculation (Table 6). But in the cv. CA-8 disease initiation was delayed up to day 8 after inoculation of the samples inelicitor treated plants where the control samples produced the initiation of lesion on the 6th day after inoculation. Moreover, a significant reduction in the disease incidence was observed in cv.CA-8 till the day 9 of disease development (Table 6). Moreover, by day 9 after inoculation, 97% of the total samples of cv. CA-8 showed the disease development in the control and however, only around 70% of the samples, obtained from the Silicon treated plants, were diseased by day 9 (Table 6). In contrast, 97% of the C. annum samples of cv. HYW, obtained from the elicitor treated plants, were diseased by day 8 after inoculation, however, results were not significant compared with the control samples (Table 7).
Table 6. Effect of Silicon soil-drench treatments (200mg/l) on severity and anthracnose disease incidence in Challenge inoculated C. annum (cv. CA-8) with C. acutatum.
Table 7. Effect of Silicon soil-drench treatments (200mg/l) on severity and anthracnose disease incidence in challenge inoculated C. annum (cv. HYW) with C. accutatum.
Table 8. Percentage reduction in anthracnose disease development of pepper cvs. CA-8 and HYW by potassium silicate (Kasil®) Soil-drench treatments.
The degree of disease reduction is the elicitor treatment in cv. CA-8 and HYW is shown in table 8. Among these, cv. CA-8 showed the greatest disease reduction that gave more than 50% protection compared to the control throughout the whole period. Disease reduction ranged from 100% to 40% from day 4 to 10 days after inoculation in cv. CA-8 whereas it was 100% to 25% in the cv. HYW.

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