Ultramicroscopy is an advanced investigative medical tool that allows to generate a three dimensional view by optical sectioning of biological specimens 8. In various literatures, the authors have discussed that Ultramicroscopy is a powerful and effective tool for 3D image reconstruction. It has proved to be a novel technique which has given new impulses in the field of biological science. It can reconstruct 3D architecture of very minute specimen of size in cm with a resolution in micrometres. And hence it has gained its importance and considered as a versatile tool for studying the anatomy of numerous biological and medical specimens. This technique is used in wide areas like viewing the neuron structure of the brain of a mouse, reconstruction of whole immuno histochemically labelled mouse embryos, giving 3D view to micro vascular networks etc.