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What exactly is germination? Germination can be defined as the process where an organism “wakes” up (“Seed Germination, Definition and Conditions,” 2018). The whole process of germination covers all the changes from the earliest sprouting of the seed till it established itself as an independent plant. In order for germination to occur there are a set number of circumstances that need to be met. Some of the circumstances include a supply of water, the optimum temperature for the seed and finally lots of oxygen. That being said there are many different methods that can be used to help increase the rate of germination. Seeds need water in order to germinate but what would happen if you used a substance such as twenty-five percent acetone? In order to see if the acetone would increase or decrease the rate of cellular respiration, an experiment was conducted. The hypothesis for this experiment was that the acetone would inhibit enzyme activity and less carbon dioxide would be produced. During this experiment two groups of seeds were tested, one being soaked in forty degrees celsius water and the other group being soaked in acetone. The acetone served as the dependent variable and the acetone was then the independent variable. In order to get accurate results three trials were conducted. During the three trials the temperature of the water, the time the spent under the CO2 sensor and the amount of peas all remained constant.

Methods and Materials:
At the start of the experiment a centrifuge tube was filled to the forty-five milliliter mark with germinating peas (Pisum sativum). The peas then were removed from the tube and placed in to a beaker containing forty degree celsius water, the beaker contained just enough water to cover the peas, they were then left to soak for ten minutes. During the ten minutes a water tube was filled with forty degree celsius water, then a sample bottle was gotten and inserted into the cross-cut lid and then attached to the water tube. During that process no water had gotten into the sample bottle. After the peas were done soaking they were drained and blotted dry and quickly transferred into the sample bottle. The CO2 sensor was then placed into the sample bottle and sealed tightly using a piece of Parafilm around the bottle and the sensor. After the sensor was inserted into the bottle, play was pressed on the Labquest and the test ran for five minutes. After the run was finished the first two columns on the graph were highlighted and then analyze was pressed and the delta y was recorded. After the data was collected the peas were then soaked in twenty five percent acetone for ten minutes, after the ten minutes were up the same procedure was repeated as was done with the peas soaked in water. This whole procedure was then repeated another two times just to ensure accurate results. A t-test was performed at the end of the experiment to further interpret the results.

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Results:
After the experiment was complete a t-test was used to get a better understanding of the results. The range between the water and the acetone was -2.776 and 2.776 was is the positive and negative values of the t two tail value. The p-value for the two tail was 0.50 which showed no significant difference between the two groups tested. Since there was only two groups that were tested, only one t-test was necessary.

Discussion:
The purpose of this lab was to see if acetone would have any effect of the cellular respiration of the peas.The hypothesis for this experiment was that the acetone would inhibit enzyme activity and less carbon dioxide would be produced. The graph supports the prediction that the acetone would slow down the rate of cellular respiration. There are a multiple number of ways this experiment could be improved. Some of these ways include making sure the peas were completely dry before putting them into the sample bottle, and to make sure the peas were soaked for exactly ten minutes. There might have been some errors that occurred during the experiment which could have affected the results, some of these errors could have been not hitting play on the Labquest to start the run, or the co2 sensor not working properly, the temperature being to high of to low. Another lab that could be done would be soaking the peas for different amounts of time to see if the time has any effect of the rate of respiration.

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